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  <titleInfo>
    <title>3D cell culture</title>
    <subTitle>methods and protocols</subTitle>
  </titleInfo>
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    <place>
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    <publisher>Humana Press/Springer</publisher>
    <dateIssued>c2011</dateIssued>
    <issuance>monographic</issuance>
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    <extent>xi, 343 p. ill. 27 cm.</extent>
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  <tableOfContents>3D cell culture : a review of current approaches and techniques / John W. Haycock -- Scaffolds for tissue engineering and 3D cell culture / Eleonora Carletti, Antonella Motta, and Claudio Migliaresi -- Tracking nanoparticles in three-dimensional tissu e-engineered models using confocal laser scanning microscopy / Vanessa Hearnden, Sheila MacNeil, and Giuseppe Battaglia -- Using immuno-scanning electron microscopy for the observation of focal adhesion-substratum interactions at the nano- and microscale in S-phase cells / Manus J. Biggs, R. Geoff Richards, and Matthew J. Dalby -- 3D sample preparation for orthopaedic tissue engineering bioreactors / Sarah H. Cartmell ... [et al.] -- Quantification of mRNA using real-time PCR and western blot analysis of MAPK events in chondrocyte/agarose constructs / David A. Lee ... [et al.] -- Genetic modification of chondrocytes using viral vectors / Teresa Coughlan ... [et al.] -- Stem cell and neuron co-cultures for the study of nerve regeneration / Paul J. Kingham, Cristina Mantovani, and Giorgio Terenghi -- Production of tissue-engineered skin and oral mucosa for clinical and experimental use / Sheila MacNeil, Joanna Shepherd, and Louise Smith -- Three-dimensional alignment of Schwann cells using hydrolysable microfiber scaffolds : strategies for peripheral nerve repair / Celia Murray-Dunning ... [et al.] -- Encapsulation of human articular chondrocytes into 3D hydrogel : phenotype and genotype characterization / Rui C. Pereira ... [et al.] -- Micro-structured materials and mechanical cues in 3D collagen gels / James B. Phillips and Robert Brown -- Organotypic and 3D reconstructed cultures of the human bladder and urinary tract / Claire L. Varley and Jennifer Southgate -- Ex vivo organ culture of human hair follicles : a model epithelial-neuroectodermal-mesenchymal interaction system / Desmond J. Tobin -- Human endothelial and osteoblast co-cultures on 3D biomaterials / Ronald E. Unger ... [et al.] -- Assessment of nanomaterials cytotoxicity and internalization / Noha M. Zaki and Nicola Tirelli -- Practical aspects of OCT imaging in tissue engineering / Stephen J. Matcher -- Osteogenic differentiation of embryonic stem cells in 2D and 3D culture / Lee Buttery ... [et al.] -- 3D structuring of biocompatible and biodegradable polymers via stereolithography / Andrew A. Gill and Frederik Claeyssens -- Alvatex? : polystyrene scaffold technology for routine three dimensional cell culture / Eleanor Knight ... [et al.]</tableOfContents>
  <note type="statement of responsibility">edited by John W. Haycock. </note>
  <note>Includes bibliographical references and index. </note>
  <subject authority="lcsh">
    <topic>Cell culture</topic>
    <topic>Technique</topic>
  </subject>
  <subject authority="mesh">
    <topic>Cell Culture Techniques</topic>
    <topic>methods</topic>
    <topic>Laboratory Manuals</topic>
  </subject>
  <classification authority="lcc">QH585.2 .A15 2011</classification>
  <classification authority="nlm">QS 525 T531 2011</classification>
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      <title>Methods in molecular biology (Clifton, N.J.) v. 695</title>
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  <relatedItem type="series">
    <titleInfo>
      <title>Springer protocols</title>
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  <identifier type="isbn">1607619830 (alk. paper)</identifier>
  <identifier type="isbn">1607619849 (e-ISBN)</identifier>
  <identifier type="isbn">9781607619833 (alk. paper)</identifier>
  <identifier type="isbn">9781607619840 (e-ISBN)</identifier>
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    <recordChangeDate encoding="iso8601">20251012174251.0</recordChangeDate>
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