000 03358nam a2200253Ia 4500
001 0000000004
005 20251012174251.0
008 101004s2011 nyua b 001 0 eng d
020 _a1607619830 (alk. paper)
020 _a1607619849 (e-ISBN)
020 _a9781607619833 (alk. paper)
020 _a9781607619840 (e-ISBN)
050 0 0 _aQH585.2
_b.A15 2011
060 1 _aQS 525
_bT531 2011
245 0 0 _a3D cell culture
_bmethods and protocols
_cedited by John W. Haycock.
260 _aNew York
_bHumana Press/Springer
_cc2011.
300 _axi, 343 p.
_bill.
_c27 cm.
504 _aIncludes bibliographical references and index.
505 0 _a3D cell culture : a review of current approaches and techniques / John W. Haycock -- Scaffolds for tissue engineering and 3D cell culture / Eleonora Carletti, Antonella Motta, and Claudio Migliaresi -- Tracking nanoparticles in three-dimensional tissu e-engineered models using confocal laser scanning microscopy / Vanessa Hearnden, Sheila MacNeil, and Giuseppe Battaglia -- Using immuno-scanning electron microscopy for the observation of focal adhesion-substratum interactions at the nano- and microscale in S-phase cells / Manus J. Biggs, R. Geoff Richards, and Matthew J. Dalby -- 3D sample preparation for orthopaedic tissue engineering bioreactors / Sarah H. Cartmell ... [et al.] -- Quantification of mRNA using real-time PCR and western blot analysis of MAPK events in chondrocyte/agarose constructs / David A. Lee ... [et al.] -- Genetic modification of chondrocytes using viral vectors / Teresa Coughlan ... [et al.] -- Stem cell and neuron co-cultures for the study of nerve regeneration / Paul J. Kingham, Cristina Mantovani, and Giorgio Terenghi -- Production of tissue-engineered skin and oral mucosa for clinical and experimental use / Sheila MacNeil, Joanna Shepherd, and Louise Smith -- Three-dimensional alignment of Schwann cells using hydrolysable microfiber scaffolds : strategies for peripheral nerve repair / Celia Murray-Dunning ... [et al.] -- Encapsulation of human articular chondrocytes into 3D hydrogel : phenotype and genotype characterization / Rui C. Pereira ... [et al.] -- Micro-structured materials and mechanical cues in 3D collagen gels / James B. Phillips and Robert Brown -- Organotypic and 3D reconstructed cultures of the human bladder and urinary tract / Claire L. Varley and Jennifer Southgate -- Ex vivo organ culture of human hair follicles : a model epithelial-neuroectodermal-mesenchymal interaction system / Desmond J. Tobin -- Human endothelial and osteoblast co-cultures on 3D biomaterials / Ronald E. Unger ... [et al.] -- Assessment of nanomaterials cytotoxicity and internalization / Noha M. Zaki and Nicola Tirelli -- Practical aspects of OCT imaging in tissue engineering / Stephen J. Matcher -- Osteogenic differentiation of embryonic stem cells in 2D and 3D culture / Lee Buttery ... [et al.] -- 3D structuring of biocompatible and biodegradable polymers via stereolithography / Andrew A. Gill and Frederik Claeyssens -- Alvatex? : polystyrene scaffold technology for routine three dimensional cell culture / Eleanor Knight ... [et al.]
650 0 _aCell culture
_xTechnique.
650 1 2 _aCell Culture Techniques
_xmethods
_vLaboratory Manuals.
830 0 _aMethods in molecular biology (Clifton, N.J.)
_vv. 695.
830 0 _aSpringer protocols.
999 _c152
_d152